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US 5,776,672 AGranted
Gene detection method
AssigneeKabushiki Kaisha Toshiba
InventorsKoji Hashimoto, Keiko Ito, Yoshio Ishimori, Masanori Gotoh
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AssigneeKabushiki Kaisha Toshiba
InventorsKoji Hashimoto, Keiko Ito, Yoshio Ishimori, Masanori Gotoh
Estimating the term…
A single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is immobilized onto the surface of an electrode or the tip of an optical fiber, and the nucleic probe is reacted with the gene sample denatured to a single stranded form, and then the nucleic acid probe hybridized with the gene is detected. In this procedure, to the reaction system consisting of the nucleic acid probe and the gene sample, a double stranded nucleic acid recognizing substance capable of binding specifically to the double stranded nucleic acid and being active electrochemically or optically is added. The detection of the nucleic acid probe is conducted by electrochemical or optical determination utilizing the electrode or optical fiber mentioned above. By this method, safer and more convenient detection of the gene is possible at a higher sensitivity even in a reduced time period.
A gene detection method wherein a single stranded nucleic acid probe having a base sequence complementary to the gene to be detected is reacted with a gene sample denatured into a single stranded form and then said nucleic acid probe hybridized with the gene is detected to confirm the presence of the gene, comprising the steps of:
Dependencies are inferred from the claim text.
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Hybridisation assays: enzymatic or biochemical coupling of nucleic acids to a solid phase
With provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples: with physical…
Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses…
Hybridisation assays: characterised by the detection means
Term ended 7 Jul 2015 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
1. Field of the Invention
This invention relates to a novel gene detection method to detect a certain gene specifically and a device for such detection.
2. Description of the Related Art
The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate a presently preferred embodiment of the invention, and together with the general description given above and the detailed description of the preferred embodiment given below, serve to explain the principles of the invention.
FIG. 1 shows a diagram schematically representing an example of the automatic gene detection device according to the present invention.
FIG. 2 shows a perspective view of another embodiment of the reaction bath and a gene sample purification device of the present automatic gene detection device shown in FIG. 1.
In the present invention, the term "double stranded nucleic acid recognizing substance" means a substance which recognizes and binds specifically to a double stranded nucleic acid. Such substances are, for example, intercalating agents and biopolymers capable of recognizing double stranded nucleic acid.
The intercalating agents are characterized by their tendency to intercalate specifically to double stranded nucleic acid such as double stranded DNA. These intercalating agents have in their molecules a flat intercalating group such as phenyl group, which intercalates between the base pairs of the double stranded nucleic acid, whereby binding to the double stranded nucleic acid. Most of the intercalating agents are optically active and some of them are used in qualification of nucleic acids. Certain intercalating agents exhibit electrode response. Therefore, determination of physical change, especially optical or electrochemical change, may serve to detect the intercalating agents bound to a double stranded nucleic acid.
Electrochemically or optically active intercalating agents useful in the present invention are, but are not limited to, ethidium, ethidium bromide, acridine, aminoacridine, acridine orange, proflavin, ellipticine, actinomycin D, daunomycin, mitomycin C, HOECHST 33342, HOECHST 33258, aclarubicin, DAPI, Adriamycin, pirarubicin, actinomycin, tris (phenanthroline) zinc salt, tris (phenanthroline) ruthenium salt, tris (phenantroline) cobalt salt, di (phenanthroline) zinc salt, di (phenanthroline) ruthenium salt, di (phenanthroline) cobalt salt, bipyridine platinum salt, terpyridine platinum salt, phenanthroline platinum salt, tris (bipyridyl) zinc salt, tris (bipyridyl) ruthenium salt, tris (bipyridyl) cobalt salt, di (bipyridyl) zinc salt, di (bipyridyl) ruthenium salt, di (bipyridyl) cobalt salt, and the like. Other intercalating agents which are useful are those listed in Published Japanese Patent Application No. 62-282599.