Genetic constructs and methods for producing fruits with very little or diminished seed
AssigneePioneer Hi-Bred International, Inc.
InventorsDwight T. Tomes, Bin Huang, Paul D. Miller
Life of this patent
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Abstract
The invention discloses a transgenic method for producing fruit with diminished or very little seeds or fruits with reduced seed number. It involves the temporal expression of a cytotoxic gene or combination of genes targeted toward arresting seed development at a time sufficiently after pollination that fruit development and maturation is normal while early enough in seed development that seed maturation per se is minimized. The invention also includes transgenic constructs, vectors, and methods for production of the fruit with diminished or very little seed bearing plants.
Claims27
An expression construct for production of transgenic plants that will produce fruit with seed of a diminished size or very little number of seed comprising:
- a recombinant gene or combination of genes which encode upon expression a cytotoxic protein;
- and
- a seed specific promoter operably linked to said gene or genes.
An expression construct for the production of a transgenic parental line which when crossed with a second parental line containing a DNA sequence which encodes a second gene product which is a non-toxic substance or encodes a second gene product which is capable of converting a substance endogenous to a plant cell to a non-toxic substance, said nontoxic substance one which is made cytotoxic by said first gene product, will produce an F1 plant which will develop fruit with seed of a diminished size or very little number of seed comprising:
- a DNA sequence which encodes a first gene product which is capable of rendering a non-toxic substance cytotoxic to a cell of a plant, said first gene product operably linked to a seed specific promoter.
An expression construct for the production of a transgenic parental line which, when crossed with a second parental line which contains a DNA sequence regulated by a seed specific promoter which encodes a first gene product which is capable of rendering a non-toxic substance cytotoxic to a cell of a plant, which will produce an F1 plant which will develop fruit with seed of a diminished size or very little number of seed comprising:
- a DNA sequence which encodes a second gene product which is a non-toxic substance or encodes a second gene product which is capable of converting a substance endogenous to a plant cell to a non-toxic substance, said nontoxic substance one which is made cytotoxic by said first gene product.
A method of producing a hybrid seed which, will produce a plant which, when pollinated will produce fruit with seed of a diminished size or very little number of seed comprising:
- pollinating a parent plant which has been transformed or which ancestor thereof has been transformed with a first DNA sequence which encodes a first gene product which is capable of rendering a non-toxic substance cytotoxic to a cell of a plant said DNA sequence operably linked to a maternal tissue specific promoter;
- and
- with a second parent plant which has been transformed or whose ancestor has been transformed with a second DNA sequence which encodes a second gene product which is a non-toxic substance o codes a second gene product which is of converting a substance endogenous to a plant cell to a non-toxic substance, said DNA sequence operably linked to a maternal tissue specific promoter, and said nontoxic substance one which is made cytotoxic by said first gene product.
A fruit with diminished or very little seed produced by a plant which contains a DNA sequence encoding a cytotoxic protein expressed during seed development.
A method of producing fruit with diminished or very little seed comprising:
- transforming an angiosperm plant cell with a DNA sequence which encodes a cytotoxic gene operably linked to a seed specific promoter;
- generating a plant from said transformed cell;
- and
- allowing said plant to be pollinated so that seed development is initiated and then halted without interrupting fruit set.
A method of producing a hybrid plant which, when pollinated will produce fruit with seed of a diminished size or very little number of seed comprising:
- pollinating a parent plant which has been transformed or which ancestor thereof has been transformed with a first DNA sequence which encodes a first gene product which is capable of rendering a non-toxic substance cytotoxic to a cell of a plant said DNA sequence operably linked to a seed specific promoter;
- and
- with a second parent plant which has been transformed or whose ancestor has been transformed with a second DNA sequence which encodes a second gene product which is a non-toxic substance or encodes a second gene product which is capable of converting a substance endogenous to a plant cell to a non-toxic substance, said DNA sequence operably linked to a seed specific promoter, and said nontoxic substance one which is made cytotoxic by said first gene product.
Dependencies are inferred from the claim text.