Antifungal polypeptide and methods for controlling plant pathogenic fungi
AssigneeMonsanto Company
InventorsJihong Liang, Dilip Maganlal Shah, Yonnie Shun Wu, Cindy Annette Rosenberger
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Abstract
An antifungal polypeptide, AlyAFP, that controls fungal damage to plants is provided. DNA encoding this polypeptide can be cloned into vectors for transformation of plant-colonizing microorganisms or plants, thereby providing a method of inhibiting fungal growth on plants. The polypeptide can be formulated into compositions that can be used to control undesired fungi on plants and elsewhere.
Claims19
A purified nucleic acid segment encoding an antifungal protein having the sequence of SEQ ID NO:2.
A recombinant, double-stranded DNA molecule, comprising the following sequences operatively linked in the 5' to 3' direction:
- a) a promoter that functions in plant cells to cause transcription of an adjacent coding sequence;
- b) a coding sequence that encodes a protein comprising an antifungal protein having the sequence of SEQ ID NO:2;
- and
- c) a 3' non-translated sequence that functions in plant cells to cause transcriptional termination and the addition of polyadenylate nucleotides to the 3' end of the transcribed RNA sequence.
A method of controlling fungal damage to a plant, comprising the steps of:
- a) inserting into the genome of plant cells a recombinant, double-stranded DNA molecule comprising the following sequences operatively linked in the 5' to 3' direction:
- (i) a promoter sequence that functions in plant cells to cause the transcription of an adjacent coding sequence;
- (ii) a coding sequence that encodes a polypeptide having the amino acid sequence of SEQ ID NO:2;
- and
- (iii) a 3' non-translated sequence that functions in plant cells to cause transcriptional termination and the addition of polyadenylation nucleotides to the 3' end of the transcribed RNA sequence;
- b) obtaining transformed plant cells;
- and
- c) regenerating from said transformed plant cells a genetically transformed plant, cells of which express an antifungal effective amount of the polypeptide.
A transonic plant, cells of which contain an antifungal effective amount of a polypeptide comprising an antifungal protein having the amino acid sequence of SE ID NO:2.
Dependencies are inferred from the claim text.