D29 shuttle phasmids and uses thereof
AssigneesAlbert Einstein College of Medicine of Yeshiva University, a Division of Yeshiva University, University of Pittsburgh
InventorsWilliam R. Jacobs, Graham F. Hatfull
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Abstract
The present invention provides a conditional shuttle phasmid constructed by inserting a cosmid into a non-essential region of the D29 mycobacteriophage which is capable of introducing DNA of interest into the chromosome of mycobacteria, especially M. tuberculosis complex organisms and other slow growing mycobacteria. The present invention provides a recombinant mycobacterium which expresses a DNA of interest incorporated into its chromosome by a conditional shuttle plasmid containing the DNA of interest. The present invention further provides a mycobacterial auxotrophic mutant and method of generating auxotrophic mutants. Finally, the present invention provides a method of inactivating a mycobacterial virulence gene.
Claims15
A purified and isolated nucleic acid encoding D29 mycobacteriophage having the sequence of SEO ID NO:
- 1.
A shuttle phasmid comprising a D29 mycobacteriophage which contains an E. coli bacteriophage lambda cosmid inserted into a non-essential region of said D29 mycobacteriophage.
Dependencies are inferred from the claim text.