Solid phase immunological assay
AssigneeNational Blood Authority
InventorMarion Lesley Scott
Life of this patent
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Abstract
A solid phase method of detection or assay of the presence or amount in a serum or plasma sample of a target antibody specific to a cell surface antigen. The sample is contacted with an immobilised preparation of cells bearing the antigen and antibody bound thereto is detected or assayed by means of an indicator comprising a binding partner for the antibody bound to labelled latex particles.
Claims21
A solid phase method of detection or assay of the presence or amount, in a serum or plasma sample, of a target antibody specific to a cell surface antigen comprising contacting the sample, suspected containing the target antibody, with an immobilised preparation of cells bearing said antigen and detecting antibody bound thereto by means of an indicator, said indicator comprising a binding partner for said antibody bound to labelled latex particles.
A kit for solid phase detection or assay of the presence or amount in a serum or plasma sample of a target antibody specific to a cell surface antigen, the kit comprising
- a) at least one cell membrane preparation presenting antigens to said target antibody, said membrane being immobilized on a solid surface;
- and
- b) a detectably labelled indicator reagent comprising a binding partner for said target antibody bound to latex particles.
Dependencies are inferred from the claim text.