Human epithelial cell matrix and uses therefor
AssigneeNorthwestern University
InventorsJonathan C. R. Jones, Stephanie Stahl, Sigmund A. Weitzman
Life of this patent
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Abstract
A method of growing epithelial cells by contacting the cells with the extracellular matrix deposited or secreted by human MCF-10A mammary epithelial cells. The MCF-10A matrix stimulates cell attachment and hemidesmosome formation in epithelial cells contacted therewith. The invention also encompasses the isolated or purified MCF-10A matrix, compositions comprising the matrix, and shaped articles coated with the matrix.
Claims23
A method of stimulating hemidesmosome formation in epithelial cells, comprising:
- contacting epithelial cells other than MCF-10A cells with the matrix protein obtainable from MCF-10A human epithelial cells, the protein having the following characteristics:
- it is capable of stimulating hemidesmosome formation;
- and
- it comprises polypeptides having molecular weights of about 100 kDa, 135 kDa and 150 kDa as determined by polyacrylamide gel electrophoresis under reducing conditions;
- whereby the epithelial cells are stimulated to attach to a substrate and produce hemidesmosomes.
A method of facilitating the growth of epithelial cells comprising:
- contacting epithelial cells other than MCF-10A cells with the matrix protein obtainable from MCF-10A human epithelial cells, the protein having the following characteristics:
- it is capable of stimulating hemidesmosome formation;
- and
- it comprises polypeptides having molecular weights of about 100 kDa, 135 kDa and 150 kDa as determined by polyacrylamide gel electrophoresis under reducing conditions;
- and
- growing the epithelial cells in contact with the matrix.
The extracellular matrix protein obtainable from MCF-10A human epithelial cells, in isolated or purified form, the protein having the following characteristics:
- it is capable of stimulating hemidesmosome formation;
- and
- it comprises polypeptides having molecular weights of about 100 kDa, 135 kDa and 150 kDa as determined by polyacrylamide gel electrophoresis under reducing conditions.
A shaped article coated with the matrix protein obtainable from MCF-10A human epithelial cells, the protein having the following characteristics:
- it is capable of stimulating hemidesmosome formation;
- and
- it comprises polypeptides having molecular weights of about 100 kDa, 135 kDa and 150 kDa as determined by polyacrylamide gel electrophoresis under reducing conditions.
A composition for use in growing epithelial cells other than MCF-10A cells comprising:
- the matrix protein obtainable from MCF-10A human epithelial cells, in isolated or purified form, the protein having the following characteristics:
- it is capable of stimulating hemidesmosome formation;
- and
- it comprises polypeptides having molecular weights of about 100 kDa, 135 kDa and 150 kDa as determined by polyacrylamide gel electrophoresis under reducing conditions;
- and
- a pharmaceutically-acceptable carrier.
Dependencies are inferred from the claim text.