Mutant E. coli strain with increased succinic acid production
AssigneeThe University of Chicago
InventorsMark Donnelly, Cynthia S. Millard, Lucy Stols
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Abstract
A method for isolating succinic acid producing bacteria is provided comprising increasing the biomass of an organism which lacks the ability to catabolize pyruvate, and then subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow. The invention also provides for a mutant that produces high amounts of succinic acid, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase, and which belongs to the E.coli Group of Bacteria.
Claims10
A method for isolating succinic acid producing bacteria comprising:
- a.) isolating a facultative organism lacking the capacity to catabolize pyruvate;
- b.) increasing the biomass of the organism in an aerobic process;
- c.) subjecting the biomass to glucose-rich medium in an anaerobic environment to enable pyruvate-catabolizing mutants to grow;
- and
- d.) isolating a mutant characterized in that it produces a mixture of succinic acid, acetic acid and ethanol as fermentation products, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase which belongs to the E. coli Group of Bacteria.
A mutant characterized in that it produces a mixture of succinic acid, acetic acid and ethanol as fermentation products, which as been derived from a parent which lacked the genes for pyruvate formate lyase and lactate dehydrogenase which belongs to the E. coli Group of Bacteria.
Dependencies are inferred from the claim text.