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US 5,770,418 AGranted
Purified polyporus laccases and nucleic acids encoding same
AssigneesNovo Nordisk A/S, Novo Nordisk Biotech, Inc.
InventorsDebbie Sue Yaver, Feng Xu, Henrik Dalb.o slashed.ge, Palle Schneider, Dorrit A. A.ae butted.lyng
Life of this patent
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Abstract
The present invention relates to isolated nucleic acid constructs containing a sequence encoding a Polyporus laccase, and the laccase proteins encoded thereby.
Claims28
A DNA cunstluct oimprising a nucleic acid sequence encoding a Polyponis laccase selected from the group consisting of:
- (a) a nucleic acid scquence which encodes the amino acid sequence of SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID NO:8, or SEQ ID NO:
- 10;
- and
- (b) a nucleic acid sequence endogenous to a Poyporus strain, which is capabic of hybridizing to (i) the nucleic acid sequence of SEQ ID NO:
- 1, SEQ ID NO:
- 3, SEQ ID NO:5, SEQ ID NO:7, or SEQ ID NO:9, or (ii) aiiy of their complementary strands, at 65.degree. C. in 1. 5.times.SSPE, 1% SDS, 0. 5% non-fat dried milk, and 200 .mu.g/ml salmon sperm DNA.
3 more elements
Dependencies are inferred from the claim text.