Platelet-specific chimeric 7E3 immunoglobulin
AssigneesResearch Foundation of the State of New York, Centocor, Inc.
InventorsBarry S. Coller, David M. Knight
Life of this patent
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Abstract
Platelet-specific, chimeric immunoglobulin and immunoglobulin fragments are described. The chimeric molecules are made up of a nonhuman antigen binding region and a human constant region. Preferred immunoglobulins are specific for glycoprotein IIb/IIIa receptor in its complexed form; they block ligand binding to the receptor and prevent platelet aggregation. The immunoglobulins are useful in anti-thrombotic therapy when administered alone or in conjunction with thrombolytic agents, as well as in thrombus imaging.
Claims21
A pharmaceutical composition comprising a chimeric immunoglobulin or chimeric immunoglobulin fragment having specificity for glycoprotein IIb/IIIa receptor and a pharmaceutically acceptable vehicle, said immunoglobulin or immunoglobulin fragment comprising an antigen binding region or a functional portion of said antigen binding region having specificity for glycoprotein IIb/IIIa receptor of the 7E3 monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832 and at least a portion of a constant region of human origin.
A pharmaceutical composition comprising a chimeric immunoglobulin fragment having specificity for glycoprotein IIb/IIIa receptor and a pharmaceutically acceptable vehicle, said immunoglobulin fragment comprising murine heavy and light chain variable regions of the monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832, a portion of a human heavy chain constant region and a human light chain constant region.
A chimeric immunoglobulin or chimeric immunoglobulin fragment comprising an antigen binding region or a functional portion of said antigen binding region having specificity for glycoprotein IIb/IIIa receptor of the 7E3 monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832 and at least a portion of a constant region of human origin.
A chimeric immunoglobulin or chimeric immunoglobulin fragment comprising:
- a) at least one chimeric heavy chain comprising a heavy chain antigen binding region or a functional portion of said antigen binding region having specificity for glycoprotein IIb/IIIa receptor of the 7E3 monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832;
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- b) at least one chimeric light chain comprising a light chain antigen binding region or a functional portion of said antigen binding region having specificity for glycoprotein IIb/IIIa receptor of the 7E3 monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832.
A chimeric immunoglobulin fragment comprising heavy and light chain antigen binding regions or a functional portion of said antigen binding region having specificity for glycoprotein IIb/IIIa receptor of the 7E3 monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832 and all or a portion of each of the human CH.sub.1 heavy chain and light chain constant regions.
A chimeric immunoglobulin Fab fragment comprising murine heavy and light chain variable regions of the monoclonal antibody produced by the hybridoma having ATCC accession number HB 8832, a portion of a human heavy chain constant region and a human light chain constant region.
A chimeric 7E3 monoclonal antibody or a chimeric 7E3 monoclonal antibody fragment, comprising at least a portion of a human constant region.
Dependencies are inferred from the claim text.