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AssigneeNovartis AG
InventorsKirsten Bojsen, Iain Donaldson, Anna Haldrup, Morten Joersboe, Jette Dina Kreiberg, John Nielsen, Finn Thyge Okkels, Steen Guldager Petersen
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The present invention provides a method for identifying or selecting from a population of eukaryotic cells cultivated on or in a medium containing at least one compound, cells which have a metabolic advantage as a result of having being transformed, wherein: i) the cells are transformed with a nucleotide sequence or a co-introduced nucleotide sequence one of which comprises a region which: (a) encodes a protein which is involved in the metabolism of the compound, and/or (b) regulates the activity of the protein; and ii) the compound is mannose or xylose or a derivative or a precursor of these, or a substrate of the protein, or is capable of being metabolized by the transformed cells into such a substrate, with the proviso that the compound is not mannose when the protein is mannose 6 phosphate isomerase. The invention also includes a method according to the preceding paragraph wherein the compounds are not so limited with the proviso that an agent which reduces the toxicity to the cells of the compound is added to the medium. It is preferred that where a toxicity-reducing agent has been added to the culture medium, the compound is mannose and the nucleotide or co-introduced nucleotide sequence encodes mannose-6-phosphate isomerase.
A method for selecting genetically transformed plant cells from a population of cells comprising:
Genetically transformed corn cells comprising,
Genetically transformed plant cells comprising an introduced nucleotide sequence and a co-introduced nucleotide sequence wherein the expression or transcription of the co-introduced nucleotide sequence gives the transformed cells a competitive advantage over non-transformed cells when both transformed and non-transformed cells in a population of cells are supplied with a compound and said transformed cells are selected based on said competitive advantage, wherein the co-introduced nucleotide sequence is a gene encoding an enzyme involved in mannose metabolism selected from the group comprising a phosphomanno isomerase, a phosphomanno mutase, a mannose epimerase, a phosphatase, and a permease.
Dependencies are inferred from the claim text.