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AssigneeHoechst Aktiengesellschaft
InventorsDenes Dudits, Sandor Morocz, Janos Nemeth, Gunter Donn
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Protoplasts which regenerate reproducibly in a short time to normal, fertile plants can be regenerated from an auxin-autotrophic genotype of Zea mays (L.). Starting from immature embryos on hormone-free media, an auxin-autotrophic, embryogenic callus is formed on the shoot basis of the seedlings, which callus retains its embryogenic potential over a substantial period of time when subcultured on hormone-free medium. In addition to fully-developed embryos, adventitious embryos are also formed under suitable culture conditions (6-9% of sucrose in the medium). When the sucrose content is reduced to 2-3% and 2,4-dichlorophenoxyacetic acid is added, soft, granular calli are formed which consist of embryogenic cell aggregates (type II callus). After subculturing the type II callus in the form of a cell suspension culture, totipotent protoplasts can be isolated. From these protoplasts, the maize plants according to the invention are regenerated.
An auxin-autotrophic transgenic Zea mays (L.) cell line from the cell line having the name and accession number DSM 6009.
An auxin-autotrophic transgenic protoplast-derived maize cell line, derived from a Zea mays (L.) genotype cell line which is auxin-autotrophic and from which protoplasts, which can reproducibly and stably regenerate into fertile plants, can be produced.
An auxin-autotrophic transgenic maize cell line, derived from protoplasts from the cell line having the name and accession number DSM 6009.
An auxin-autotrophic fertile transgenic Zea mays (L.) plant having cells which can be cultured and maintained on auxin-free media.
A transgenic auxin-autotrophic Zea mays (L.) cell line from which fertile plants can be obtained.
Dependencies are inferred from the claim text.