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US 5,766,920 AGranted
Ex vivo activation of immune cells
AssigneeCellcor, Inc.
InventorsBruce P. Babbitt, Zhengyi J. Zhang
Life of this patent
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Abstract
Disclosed is a process of activating patient-derived mononuclear cells by exposing the cells in vitro to substances wo generate immunoreactive cells. The ex vivo activated cells are then reinfused into the patient to enhance the immune system to treat various forms of cancer, infectious diseases, autoimmune diseases or immune deficiency diseases.
Claims17
A process of producing a population of immunoreactive cells comprising the steps of:
- (a) contacting a first sample of mononuclear cells derived from a patient with OKT3 at or below 37.degree. C. to produce an OKT3-derived culture supernatant (T3CS);
- (b) removing said T3CS from said first sample;
- (c) determining the concentration of OKT3 in said T3CS, and if required, supplementing said T3CS with said OKT3 to achieve a concentration in the range of 1-25 ng/ml;
- (d) providing a second sample of mononuclear cells derived from said patient;
- and
- (e) contacting said second sample with said T3CS and said OKT3 for a period of time sufficient to activate said second sample in vitro to yield a population of immunoreactive cells.
3 more elements
A process of producing a population of antigen-specific polyclonal T cells comprising the steps of:
- (a) contacting a first sample of mononuclear cells derived from a patient with OKT3 at or below 37.degree. C. to produce a T3CS;
- (b) removing said T3CS from said first sample;
- (c) determining the concentration of OKT3 in said T3CS, and if required, supplementing said T3CS with said OKT3 to achieve a concentration in the range of 0.1-25 ng/ml;
- (d) providing a second sample of mononuclear cells derived from said patient;
- (e) contacting said second sample with said T3CS and an antigen for a period of time sufficient to activate said second sample in vitro to yield a population of antigen-specific polyclonal T cells.
2 more elements
Dependencies are inferred from the claim text.