US 5,766,840 A · Hepatitis G virus and molecular cloning thereof

Abstract

Polypeptide antigens are disclosed which are immunoreactive with sera from individuals having a non-A, non-B, non-C, non-D, non-E Hepatitis, herein designated Hepatitis G Virus (HGV). Corresponding genomic-fragment clones containing polynucleotides encoding the open reading frame sequences for the antigenic polypeptides are taught. The antigens are useful in diagnostic methods for detecting the presence of HGV in test subjects. The antigens are also useful in vaccine and antibody preparations. In addition, the entire coding sequences of two HGV isolates are disclosed. Methods are presented for nucleic acid-based detection of HGV in samples and also methods for the isolation of further genomic sequences corresponding to HGV.

Claims12

Dependent claims

A purified antibody preparation made against a polypeptide of SEQ ID NO:

15 or a fragment thereof which is specifically immunoreactive with Non-A Non-B Non-C Non-D Non-E Hepatitis G Virus (HGV), where HGV is characterized by the following:
(i) elevated serum alanine aminotransferase levels in an infected primate, (ii) serologic distinctness from hepatitis A virus (HAV), hepatitis B virus (HBV), hepatitis C virus (HCV), hepatitis D virus (HDV) and hepatitis E virus (HEV), and (iii) a viral genome comprising a polynucleotide region that is selectively hybridizable with SEQ ID NO:19.

Dependencies are inferred from the claim text.

Facts

Application

08/466,033Filed 5 Jun 1995 · division

Priority

20 May 1994

Granted

16 Jun 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification9 codes

C07K 14/005
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from viruses
A61K 38/00
Medicinal preparations containing peptides
C12N 2770/24222
Hepacivirus, e.g. hepatitis C virus, hepatitis G virus: new viral proteins or individual genes, new structural or functional aspects of known…
C12N 2770/36022
Togaviridae: new viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes

Family

Serial246,985Parent of this continuation-in-part · filed 20 May 1994

Serial285,558Parent of this continuation-in-part · filed 3 Aug 1994

Serial285,543Parent of this continuation-in-part · filed 3 Aug 1994

Serial285,561Parent of this continuation-in-part · filed 3 Aug 1994

Serial329,729Parent of this continuation-in-part · filed 26 Oct 1994

Serial344,271Parent of this continuation-in-part · filed 23 Nov 1994

Serial357,509Parent of this continuation-in-part · filed 16 Dec 1994

Serial389,886Parent of this continuation-in-part · filed 15 Feb 1995

Serial444,733Parent of this division · filed 19 May 1995

US 5,766,840 AThis patent

From the US filing history. Foreign family members aren’t listed.

Hepatitis G virus and molecular cloning thereof

Life of this patent

Hepatitis G virus and molecular cloning thereof

Life of this patent

Abstract

Claims12