US 5,763,583 A · Process for producing solubilized protein

Abstract

A process for the production of a solubilized protein is disclosed, which is obtained by reducing disulfide bonds in a disulfide bond-containing water-insoluble protein material into mercapto groups and subsequently converting a part or an entire portion thereof into carboxymethyldisulfide groups. In particular, the disclosed process for the production of a solubilized protein which comprises (a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent, and (b) reacting the protein treated by step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition. A process for the production of regenerated protein products, which comprises regenerating disulfide bonds in the solubilized protein, is also disclosed.

Claims5

Dependent claims

A process for the production of a solubilized protein which consists essentially of:

(a) treating a disulfide bond-containing water-insoluble protein material with an aqueous alkaline solution of a reducing agent and then treating with a protein denaturing agent;
and
(b) reacting said disulfide bond-containing water-insoluble protein material from step (a) with thioglycolic acid in the presence of an oxidizing agent under a weakly acidic to a weakly alkaline condition to convert disulfide bonds in said disulfide bond-containing water-insoluble protein material into carboxymethyldisulfide groups and thereby produce said solubilized protein, said process being conducted without any water washing step.

Dependencies are inferred from the claim text.

Process for producing solubilized protein

Life of this patent

Process for producing solubilized protein

Life of this patent

Abstract

Claims5