Gene transfer into bone cells and tissues
AssigneeThe Regent of the University of Michigan
InventorsJeffrey Bonadio, Steven A. Goldstein
Life of this patent
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Abstract
Disclosed are methods, compositions and devices for use in transferring nucleic acids into bone cells in situ. The transfer of an osteotropic gene into bone progenitor cells is described, which event is shown to stimulate the progenitor cells and to promote bone growth, repair and regeneration in vivo. These gene transfer protocols are suitable for use in transferring various nucleic acid materials into bone, and have many uses, for example, in treating various bone-related diseases and defects, such as, in promoting fracture repair, use in connection with implants, and in treating osteoporosis and osteogenesis imperfecta.
Claims77
A method for transferring a nucleic acid segment into bone progenitor cells located within a bone progenitor tissue site of an animal, comprising contacting said tissue site with a composition comprising a nucleic acid segment and a structural bone-compatible matrix so as to transfer said nucleic acid segment into said cells, wherein said nucleic acid segment expresses a transcriptional or translational product in said cells.
A method of stimulating bone progenitor cells located within a bone progentor tissue site of an animal, comprising contacting said tissue site with a composition comprising an osteotropic gene and a structural bone-compatible matrix so as to promote expression of said gene in said cells.
A composition comprising a nucleic acid segment in association with a structural bone-compatible matrix.
A composition comprising an osteotropic gene in association with a structural bone-compatible matrix, said composition being capable of stimulating bone growth when administered to a bone progenitor tissue site of an animal.
An osteotropic device, comprising an osteotropic gene capable of expression in bone progenitor cells, the gene associated with an amount of a structural bone-compatible matrix effective to absorb said gene, wherein said device is capable of stimulating bone formation when implanted within a bone progenitor tissue site of an animal.
A method of delivering a nucleic acid segment to a fibroblast cell located within a repair tissue site of an animal, comprising contacting said tissue site with a composition comprising a nucleic acid segment and a structural bone-compatible matrix to effect uptake of the nucleic acid segment into the fibroblast cell and to promote expression of a transcriptional or translational product in said cell.
A method of delivering at least one selected nucleic acid segment to a fibroblast cell located within a repair tissue site of an animal, comprising the steps of:
- (a) preparing a matrix-nucleic acid composition comprising at least one nucleic acid segment and a structural bone-compatible matrix;
- and
- (b) contacting said repair tissue site with the matrix-nucleic acid composition to effect uptake of the nucleic acid segment by the fibroblast cell wherein said nucleic acid segment expresses a transcriptional or translational product in said cell.
Dependencies are inferred from the claim text.