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AssigneeThe Regents of the University of Michigan
InventorsBernhard O. Palsson, Stephen G. Emerson, Richard M. Schwartz
Estimating the term…
Methods, compositions and devices are provided for the growth of human stem and/or hematopoietic cells in culture. Bioreactors are provided in which diverse cell types are simultaneously-cultured in the presence of appropriate levels of nutrients and growth factors substantially continuously maintained in the bioreactor while removing undesirable metabolic products. This simultaneous culture of multiple cell types successfully reconstructs hematopoietic tissue ex vivo. Optionally, at least one growth factor is provided through excretion by transfected stromal cells, particularly heterologous cells. The stromal cells and hematopoietic cells may be maintained separately, and both the adherent and non-adherent cells harvested.
A bioreactor suitable for culturing human cell and/or tissue, including human stem cells or human hematopoietic cells, comprising:
A human tissue, including human cells and/or stem cell or human hematopoietic cell expansion system, comprising:
The system of claim 29, said bioreactor including a gas permeable and water impermeable membrane fitted into said enclosure so as to divide said enclosure into said cell culture chamber, at one side, and a gas chamber, at the other side of said membrane, and means for perfusing respiratory gases through said cell culture comprising means for delivering respiratory gases to said gas chamber whereby said respiratory gases pass through said membrane and thereby penetrate said cell culture chamber, and means for withdrawing spent respiratory gases from said gas chamber.
Dependencies are inferred from the claim text.