Production and use of normalized DNA libraries
AssigneeDiversa Corporation
InventorsJay M. Short, Eric J. Mathur
Life of this patent
Estimating the term…
Abstract
Disclosed is a process for forming a normalized genomic DNA library from an environmental sample by (a) isolating a genomic DNA population from the environmental sample; (b) analyzing the complexity of the genomic DNA population so isolated; (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic; and (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample. Also disclosed is a normalized genomic DNA library formed from an environmental sample by the process.
Claims14
A process for forming a normalized genomic DNA library from an environmental sample, which comprises the steps of:
- (a) isolating a genomic DNA population from the environmental sample;
- (b) analyzing the complexity of the genomic DNA population so isolated;
- (c) at least one of the steps selected from the group consisting of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic;
- and
- (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
A normalized genomic DNA library formed from from an environmental sample by a process comprising the steps of:
- (a) isolating a genomic DNA population from the environmental sample;
- (b) analyzing the complexity of the genomic DNA population so isolated;
- (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic;
- and
- (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
A process for forming a normalized library of genomic gene clusters from an environmental sample which comprises
- (a) isolating a genomic DNA population from the environmental sample;
- (b) analyzing the complexity of the genomic DNA population so isolated;
- (c) at least one of (i) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic;
- and
- (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
A normalized library of genomic gene clusters formed from an environmental sample by a process comprising the steps of
- (a) isolating a genomic DNA population from the environmental sample;
- (b) analyzing the complexity of the genomic DNA population so isolated;
- (c) at least one of (I) amplifying the copy number of the DNA population so isolated and (ii) recovering a fraction of the isolated genomic DNA having a desired characteristic;
- and
- (d) normalizing the representation of various DNAs within the genomic DNA population so as to form a normalized library of genomic DNA from the environmental sample.
Dependencies are inferred from the claim text.