Gap gene sequences
AssigneeChiron Corporation
InventorsGail L. Wong, George Martin, Francis P. McCormick, Bonnee Rubinfeld, Edward C. O'Rourke, Robin Clark
Life of this patent
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Abstract
Guanosine triphosphatase activating protein (GAP) DNA sequences are described that are useful as cancer diagnostics, particularly to detect cancer cells that express the ras oncogene protein p21 by measuring the level of GAP gene expression or amplification.
Claims39
An isolated DNA comprising the contiguous nucleotide sequence shown in SEQ ID NO:
- 7, said contiguous nucleotide sequence encoding a polypeptide possessing GAP activity.
An isolated DNA comprising the nucleotide sequence of Clone Sleepy having ATCC No. 40504.
An isolated DNA encoding a polypeptide possessing GAP activity, said DNA comprising a contiguous portion of the nucleotide sequence shown in SEQ ID NO:
- 7, said portion encoding a polypeptide possessing GAP activity.
An isolated DNA comprising a contiguous, polypeptide-encoding insert of Clone 16 having ATCC No. 40503, said polypeptide having GAP activity.
An isolated DNA encoding a polypeptide possessing GAP activity, wherein the polypeptide has a molecular weight of about 115,000-120,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis run under reducing conditions.
An isolated DNA encoding a polypeptide possessing GAP activity and operatively linked to control sequences which regulate the synthesis of said DNA and the expression of said polypeptide in transformed host cells.
An isolated DNA encoding a polypeptide having the amino acid sequence shown in SEQ ID NO:
- 8, said polypeptide possessing GAP activity.
A baculovirus transfer vector, wherein said vector is pAcC12 GAP 5 having ATCC No. 67821.
An isolated DNA comprising a contiguous, polypeptide-encoding insert of pAcC12 GAP 5 having ATCC No. 67821, wherein said polypeptide possesses GAP activity.
An isolated DNA encoding a polypeptide possessing GAP activity, said polypeptide having a contiguous amino acid sequence corresponding to amino acid residues 181 to 1047 of SEQ ID NO:
- 8.
An isolated DNA having the contiguous nucleotide sequence corresponding to nucleotides 1 to 3456 of SEQ ID NO:
- 24.
An isolated DNA encoding a polypeptide possessing GAP activity, said DNA comprising a contiguous nucleotide sequence corresponding to nucleotides 43 to 2709 of SEQ ID NO:
- 24.
An isolated and GAP-specific polynucleotide comprising a contiguous portion of at least 14 nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 7 and the sequence complementary to SEQ ID NO:
- 7, said isolated polynucleotide capable of specifically hybridizing under high stringency hybridization conditions to a DNA encoding a polypeptide possessing GAP activity, or to the non-coding strand complementary to said DNA.
An isolated polynucleotide comprising a contiguous portion of at least 18 nucleotides of a nucleotide sequence selected from the group consisting of SEQ ID NO:
- 7 and the sequence complementary to SEQ ID NO:
- 7, said isolated polynucleotide capable of hybridizing under moderately stringent conditions to a DNA encoding a polypeptide possessing GAP activity, or to the non-coding strand complementary to said DNA, said moderately stringent hybridization conditions comprising a hybridization temperature of 42.degree. C., a hybridization buffer comprising 50% formamide, and a hybridization period of 24-36 hours.
An isolated polynucleotide comprising at least 14 nucleotides and capable of hybridizing under stringent hybridization conditions to a DNA encoding a polypeptide possessing GAP activity, or to the non-coding strand complementary to said DNA, said DNA having the sequence set forth in SEQ ID NO:
- 7.
An isolated DNA encoding a polypeptide possessing GAP activity, said DNA capable of hybridizing under stringent hybridization conditions to a polynucleotide selected from the group consisting of a DNA having the nucleotide sequence shown in SEQ ID NO:
- 7, a DNA having the nucleotide sequence of the non-coding strand complementary to SEQ ID NO:
- 7, a DNA having the nucleotide sequence shown in SEQ ID NO:
- 24, and a DNA having the nucleotide sequence of the non-coding strand complementary to SEQ ID NO:
- 24.
Dependencies are inferred from the claim text.