Calibrator and use thereof in an immunoassay
AssigneeAkzo Nobel N.V.
InventorWillem Nieuwenhuizen
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Abstract
The invention relates to a composition comprising among others a fibrinopeptide A releasing compound. Furthermore the invention relates to the use of the composition as calibrator in plasma containing fibrinogen. A test kit comprising the said composition and a method to determine soluble fibrin also belong to the invention.
Claims9
A method for preparing a composition containing a known amount of soluble fibrin, which is substantially free of fibrinogen, comprising
- (a) diluting normal plasma containing a known fibrinogen concentration with a buffer comprising a sugar alcohol, a sodium bromide, an amino acid, and a preservative;
- and
- (b) treating the diluted plasma with a fibrinopeptide A releasing compound for two hours, whereby all fibrinogen in the plasma is converted to soluble fibrin.
A composition comprising sodium bromide, a sugar alcohol, an amino acid, a preservative, a fibrinopeptide A releasing compound, and a known amount of soluble fibrin substantially free of fibrinogen, the composition made by the method of
- diluting normal plasma containing a known fibrinogen concentration with a buffer comprising sodium bromide, the sugar alcohol, the amino acid, and the preservative, and
- treating the diluted plasma with the fibrinopeptide A releasing compound for two hours, whereby all fibrinogen in the plasma is converted to soluble fibrin.
Dependencies are inferred from the claim text.