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AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsTomoyuki Nishimoto, Hiroto Chaen, Toshiyuki Sugimoto, Toshio Miyake
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Microorganisms which are able to produce maltose/trehalose conversion enzyme, a novel enzyme, are cultivated in nutrient culture media with malose. During the cultivation, the microorganisms readily convert maltose into trehalose to accumulate trehalose in the cultures which yield saccharide mixtures with high trehalose contents when separated from insoluble substances. Removal of contaminant saccharides and subsequent crystallization readily yield trehalose in crystalline trehalose hydrate or anhydrous crystalline form. The trehalose and saccharide mixture containing the same commonly bear desirable properties including mild sweetness and superior stability which render them very useful in a variety of compositions indlucing food products, cosmetics and medicines.
A trehalose-containing composition consisting essentially of trehalose, maltose, and glucose, which is obtained by cultivating in a nutrient culture containing maltose a microorganism capable of producing a maltose/trehalose conversion enzyme, separating the microorganism from the culture to obtain the nutrient culture medium free of said microorganism, and purifying the resulting nutrient culture medium to obtain said trehalose-containing composition,
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