Inhibition of cell proliferation using antisense oligonucleotides
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsStephen E. Epstein, Edith H. Speir, Ellis F. Unger
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Abstract
The present invention provides a method of inhibiting restenosis of a blood vessel in a mammal after mechanical treatment of the vessel to reduce a stenosis. The method includes contacting proliferating smooth muscle cells in the vessel with an antisense oligonucleotide directed against a cellular division cycle gene product. This gene product can be one of the following: c-myc, PCNA, and cyclin B.sub.1. The antisense oligonucleotide is used in an amount effective to inhibit translation of the cellular division cycle gene product in the cell.
Claims19
A method of inhibiting the growth of a human smooth muscle cell, comprising contacting said cell with a synthetic antisense oligonucleotide directed against a c-myc gene product in an amount effective to inhibit translation of said c-myc gene product in said cell.
A method of inhibiting the migration of a human smooth muscle cell, comprising contacting said cell with a synthetic antisense oligonucleotide directed against a c-myc gene product in an amount effective to inhibit translation of said c-myc gene product in said cell.
A method of inhibiting restenosis of a blood vessel in a human after mechanical treatment of the vessel to reduce a stenosis, the method comprising contacting the vessel with a synthetic antisense oligonucleotide directed against a c-myc gene product in an amount effective to inhibit translation of said c-myc gene product in the cells of the vessel.
Dependencies are inferred from the claim text.