DNA coding for a human vasoconstrictive peptide and use thereof
AssigneeTakeda Chemical Industries, Ltd.
InventorsHaruo Onda, Shoichi Ohkubo, Takuo Kosaka
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Abstract
Disclosed are (1) a DNA containing a cDNA segment coding for human endothelin-2 (SEQ ID NO:1), (2) a precursor of human endothelin-2 (SEQ ID NO:2), (3) a transformant carrying a DNA containing a cDNA segment coding for human endothelin-2, and (4) a method for preparing mature human endothelin-2 which comprises culturing the transformant described in (3), accumulating a protein in a culture medium, and collecting the same, whereby human endothelin-2 and the precursors thereof can be produced in large amounts.
Claims3
An isolated and purified precursor protein, which is represented by an amino acid sequence as defined in the Sequence Listing as SEQ ID NO:8.
An isolated and purified cDNA segment in which said cDNA segment is represented by the nucleotide sequence of Nos. 203 to 316 of SEQ ID NO:1.
A transformant carrying a DNA containing a cDNA segment coding for human endothelin-2 in which said cDNA segment coding for human endothelin-2 contains a nucleotide sequence represented by the nucleotide sequence of Nos. 203 to 316 of SEQ ID NO:1.
Dependencies are inferred from the claim text.