Method to deliver compositions conferring resistance to alopecia to hair follicles

A method to introduce a composition which contains at least one active ingredient which is effective to inhibit the loss of hair caused by a chemotherapeutic agent selectively to the hair follicles of a subject without transmission to the cells external to the hair follicles so as to prevent hair loss induced by chemotherapy in said subject in need of such prevention which method comprises

• applying to the skin of said subject in an area where prevention of hair loss is desired and which contains hair follicles, a formulation comprising said composition wherein said at least one active ingredient, when contained in the cells of a hair follicle, prevents the chemotherapeutic agent-induced loss of hair extruded from said hair follicle,
• said formulation being a liposomal formulation wherein said liposomes target said composition selectively into the hair follicle cells by transfer into the follicle without entry into the circulation or the adjacent skin tissue and
• wherein said active ingredient is a protein which is a cell cycle inhibitor, or
• wherein said active ingredient is the protein product of the multidrug resistance (MDR) gene, or
• wherein said composition comprises an expression system for the production of an effective amount of a protein which is a cell cycle inhibitor, or
• wherein said composition comprises an expression system for the production of an effective amount of the product of the MDR gene.