US 5,753,093 AGranted

Sensor membranes

AssigneesAustralian Membrane and Biotechnology Research Institute, The University of Sydney

InventorsBurkhard Raguse, Bruce A. Cornell, Vijoleta L. Braach-Maksvytis, Ronald J. Pace

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Abstract

The present invention relates to a novel method for producing an electrode membrane combination. This novel method employs compounds including a linker lipid for use in attaching a membrane including a plurality of ionophores to an electrode and providing a space between the membrane and the electrode, the electrode being either in part or totally made up of the linker lipid. The linker lipid comprises within the same molecule a hydrophobic region capable of spanning the membrane, an attachment group used to attach the molecule to an electrode surface, a hydrophilic region intermediate said hydrophobic region and the attachment group and a polar head group region attached to the hydrophobic region at a site remote from the hydrophilic region.

Claims24

Dependent claims

A method of producing an electrode membrane combination, the method comprising the following sequential steps:

(1) Forming a solution comprising ion channels having attached at an end thereof a reservoir region, the reservoir region including a hydrophilic group and an attachment group;
and a reservoir lipid, the reservoir lipid comprising a hydrophobic region, a hydrophilic region and an attachment region in a polar carrier solvent;
(2) Contacting the electrode with the solution from step (1), the composition of the electrode and the attachment groups and the attachment regions being selected such that the attachment groups and the attachment regions chemisorb to the electrode;
(3) After a period of incubation rinsing the coated electrode from step (2) to remove unbound material;
(4) Adding to the rinsed electrode from step (3) a solution comprising ion channels and a lipid in a carrier solvent;
and
(5) Adding to the electrode from step (4) an aqueous solution such that a lipid bilayer membrane coating the electrode is formed.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 08/833,782Filed 9 Apr 1997 · division
Priority: AU PL50691 Oct 1992
Granted: 19 May 1998
Term ends: Not estimatedThe source records don’t support an estimate.

Classification16 codes

G01N 33/54373
Apparatus specially adapted for solid-phase testing: involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
B01D 69/122
Composite membranes; Ultra-thin membranes: separate manufacturing of ultra-thin membranes
C07F 9/10
Esters of phosphoric acids: phosphatides, e.g. lecithin
C12Q 1/002
Enzyme electrodes: electrode membranes