Plant genetic transformation methods and transgenic plants

A method for producing a stably transformed raspberry or strawberry plant, comprising:

• (i) introducing into cells of explant tissue from a target raspberry or strawberry plant to be transformed, an expression vector containing a selectable marker gene operably linked to a plant compatible-promoter,
• (ii) culturing said tissues from (i) in selection media having a threshold concentration of selective agent effective to discriminate between plant cells transformed with the vector and non-transformed cells, based on their ability to grow in the media,
• (iii) selecting tissues based on their growth characteristics in the selection media,
• (iv) regenerating the selected tissues in the presence of a selective agent whose concentration is effective to discriminate between transformed and non-transformed plant cells, based on the cells's ability to produce regenerated tissue,
• (v) selecting transformed regenerated tissue generated in (iv),
• (vi) obtaining an explant from the selected, transformed regenerated tissue, and
• (vii) repeating steps (iv) and (v) at least one time, until stably transformed regenerated tissue is obtained, where step (vi) precedes each repeat cycle.