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US 5,750,397 AGranted
Human hematopoietic stem cell
AssigneeSystemix, Inc.
InventorsAnn Tsukamoto, Charles M. Baum, Yukoh Aihara, Irving Weissman
Life of this patent
Estimating the term…
Abstract
Human hematopoietic stem cells are provided by separation of the stem cells from dedicated cells. The stem cells may than be maintained by regeneration in an appropriate growth medium. Means are provided for assaying for the stem cells as to their capability for producing members of each of the hematopoietic lineages.
Claims7
A culture comprising:
- human hematopoietic stem cells having fewer than 5% lineage committed cells, wherein said human stem cells are Thy-1.sup.+, CD34.sup.+, capable of self regeneration, and capable of differentiation to members of the lymphoid, erythroid and myelomonocytic lineages;
- and
- a medium capable of supporting the growth of said stem cells.
A coculture comprising:
- a medium capable of supporting the growth of hematopoietic stem cells;
- stromal cells;
- and
- human hematopoietic cells, including stem cells and differentiated cells, wherein said coculture originated from a cellular composition comprising human hematopoletic stem cells having fewer than 5% lineage committed cells, wherein said human hematopoietic stem cells are Thy-1.sup.+, CD34.sup.+, capable of self regeneration, and capable of differentiation to members of the lymphoid, erythroid and myelomonocytic lineages.
1 more element
Dependencies are inferred from the claim text.