Methods of producing secreted receptor analogs and biologically active dimerized polypeptide fusions

A method for producing a secreted PDGF receptor analog, comprising:

• introducing into a cultured rodent myeloma cell a DNA construct comprising a transcriptional promoter operatively linked to a PDGF receptor signal sequence followed downstream by and in proper reading frame with a DNA sequence encoding the amino acid sequence of FIGS. 11A-11D (Sequence ID Numbers 35 and 36) from glutamine, number 24, to glutamic acid, number 524, joined to a dimerizing protein, wherein said dimerizing protein is an immunoglobulin constant region selected from the group consisting of C.sub.H 1, C.sub.H 2, C.sub.H 3, and C.sub.H 4 and C.sub..kappa. joined to an immunoglobulin hinge region;
• growing said cultured rodent myeloma cell in an appropriate growth medium under physiological conditions to allow the secretion of a PDGF receptor analog encoded by said DNA sequence;
• and
• isolating the PDGF receptor analog from said cultured rodent myeloma cell.

A method for producing a secreted PDGF receptor analog, comprising:

• introducing into a cultured rodent myeloma cell a first DNA construct comprising a transcriptional promoter operatively linked to a PDGF receptor signal sequence followed downstream by and in proper reading frame with a first DNA sequence encoding the amino acid sequence of FIGS. 1A-1D (Sequence ID Numbers 1 and 2) from isoleucine, number 29, to lysine, number 531 joined to an immunoglobulin light chain constant region;
• introducing into said cultured rodent myeloma cell a second DNA construct comprising a transcriptional promoter operatively linked to a PDGF receptor signal sequence followed downstream by and in proper reading frame with a second DNA sequence encoding the amino acid sequence of FIGS. 1A-1D (Sequence ID Numbers 1 and 2) from isoleucine, number 29, to lysine, number 531 joined to an immunoglobulin heavy chain constant region domain selected from the group consisting of C.sub.H 1, C.sub.H 2, C.sub.H 3, and C.sub.H 4 joined to an immunoglobulin hinge region;
• growing said cultured rodent myeloma cell in an appropriate growth medium under physiological conditions to allow the secretion of a PDGF receptor analog encoded by said first and second DNA sequences;
• and
• isolating said PDGF receptor analog from said cultured myeloma cell.

A method for producing a secreted PDGF receptor analog, comprising:

• introducing into a cultured rodent myeloma cell a first DNA construct comprising a mouse V.sub.H promoter operatively linked to a PDGF receptor signal sequence followed downstream of and in proper reading frame with a DNA sequence encoding the amino acid sequence of FIGS. 11A-11D (Sequence ID Numbers 35 and 36) from glutamine, number 24 to glutamic acid, number 524, joined to an immunoglobulin heavy chain constant region domain selected from the group consisting of C.sub.H 1, C.sub.H 2, C.sub.H 3, and C.sub.H 4 joined to an immunoglobulin hinge region;
• introducing into said cultured rodent myeloma cell a second DNA construct comprising a mouse V.sub..kappa. promoter operatively linked to a PDGF receptor signal sequence followed downstream of and in proper reading frame with a DNA sequence encoding the amino acid sequence of FIGS. 11A-11D (Sequence ID Numbers 35 and 36) from glutamine, number 24 to glutamic acid, number 524, joined to an immunoglobulin light chain constant region;
• growing said cultured rodent myeloma cell in an appropriate growth medium under physiological conditions to allow the secretion of a PDGF receptor analog encoded by said first and second DNA sequences;
• and
• isolating said PDGF receptor analog from said cultured myeloma cell.

A method for producing a secreted PDGF receptor analog, comprising:

• introducing into a cultured rodent myeloma cell a first DNA construct comprising a mouse V.sub.H promoter operatively linked to a PDGF receptor signal sequence followed downstream of and in proper reading frame with a DNA sequence encoding the amino acid sequence of FIGS. 1A-1D (Sequence ID Numbers 1 and 2) from isoleucine, number 29, to lysine, number 531, joined to an immunoglobulin heavy chain constant region domain selected from the group consisting of C.sub.H 1, C.sub.H 2, C.sub.H 3, and C.sub.H 4 joined to an immunoglobulin hinge region;
• introducing into said cultured rodent myeloma cell a second DNA construct comprising a mouse V.sub..kappa. promoter operatively linked to a PDGF receptor signal sequence followed downstream of and in proper reading frame with a DNA sequence encoding the amino acid sequence of FIGS. 11A-11D (Sequence ID Numbers 35 and 36) from glutamine, number 24 to glutamic acid, number 524, joined to an immunoglobulin light chain constant region;
• growing said cultured rodent myeloma cell in an appropriate growth medium under physiological conditions to allow the secretion of a PDGF receptor analog encoded by said first and second DNA sequences;
• and
• isolating said PDGF receptor analog from said cultured myeloma cell.