# US 5,750,101 A · Ovo immunization of avian embryos with oil-emulsion vaccines

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- Number: US 5,750,101 A
- Document ID: US5750101A
- Status: Granted
- Kind: A
- Application number: 08/530,698
- Filed: 1995-09-15
- Granted: 1998-05-12
- Earliest claimed: 1994-06-30
- Assignees: The United States of America as represented by the Secretary of Agriculture
- Inventors: Henry D. Stone
- Web page: https://patentgrep.com/patent/US5750101A

## CPC

- A61K 39/17: Newcastle disease virus
- A61K 39/0275: {Salmonella}
- A61K 39/12: Viral antigens
- A61K 2039/521: {inactivated (killed)}
- A61K 2039/54: {characterised by the route of administration}
- A61K 2039/55: {characterised by the host/recipient, e.g. newborn with maternal antibodies}
- A61K 2039/552: {Veterinary vaccine}
- A61K 2039/55566: {Emulsions, e.g. Freund's adjuvant, MF59}
- A61K 2039/70: {Multivalent vaccine}
- C12N 2720/12034: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2760/16134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C12N 2760/18134: Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- Y02A 50/30: Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
- A61K: PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES (devices or methods specially adapted for bringing pharmaceutical products into particular physical or administering forms A61J3/00; chemical aspects of, or use of materials for deodorisation of air, for disinfection or sterilisation, or for bandages, dressings, absorbent pads or surgical articles A61L; soap compositions C11D)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y02A: TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE

## Abstract

Avian diseases, particularly those which threaten birds early in life, are controlled by embryonal vaccination using oil emulsion vaccines. The site of inoculation is the albumin end of the egg via entry through the air cell end of the egg.

## Claims

### Claim 1

A method for immunizing avian embryos comprising
puncturing an egg containing said avian embryo with a puncturing means at the longitudinal midline over the egg's air cell in order to administer an oil emulsion vaccine in said egg's albumin end,
injecting said vaccine into the egg by vertically inserting a needle means through said air cell to the albumin end,
administering said vaccine into the albumin end of the egg, and
hatching said egg to obtain an immunized avian species.

### Claim 2 (depends on 1)

The method of claim 1 wherein said egg is selected from the group consisting of chicken eggs, turkey eggs, geese eggs, duck eggs, and pheasant eggs.

### Claim 3 (depends on 1)

The method of claim 1 wherein said vaccine confers immunity against microbial diseases.

### Claim 4 (depends on 1)

The method of claim 1 wherein said vaccine is selected from the group consisting of monovalent and polyvalent vaccines.

### Claim 5 (depends on 3)

The method of claim 3 wherein said diseases include Newcastle's disease, avian influenza, avian leukosis, infectious bursal disease, adenovirus disease, reovirus, pox, laryngotracheitis, infectious bronchitis, reticuloendotheliosis, infectious coryza, fowl typhoid, fowl cholera, Salmonella, and Marek's disease.

### Claim 6 (depends on 1)

The method of claim 1 wherein said oil emulsion vaccine is a mineral oil emulsion vaccine.

### Claim 7 (depends on 1)

The method of claim 1 wherein said needle means is a needle of a gauge selected from the group consisting of 27 gauge, 25 gauge, 23 gauge, 22 gauge, 20 gauge, 18 gauge, and 16 gauge.

### Claim 8 (depends on 7)

The method of claim 7 wherein said egg is selected from the group consisting of chicken eggs, turkey eggs, geese eggs, duck eggs, and pheasant eggs.

### Claim 9 (depends on 7)

The method of claim 7 wherein said vaccine is an oil emulsion vaccine.

### Claim 10 (depends on 7)

The method of claim 7 wherein said vaccine is selected from the group consisting of monovalent and polyvalent vaccines.

### Claim 11 (depends on 7)

The method of claim 7 wherein said vaccine confers immunity against microbial diseases.

### Claim 12 (depends on 11)

The method of claim 11 wherein said diseases include Newcastle's disease, avian influenza, avian leukosis, infectious bursal disease, adenovirus disease, reovirus, pox, laryngotracheitis, infectious bronchitis, reticuloendotheliosis, infectious coryza, fowl typhoid, fowl cholera, Salmonella, and Marek's disease.

### Claim 13 (depends on 9)

The method of claim 9 wherein said oil emulsion vaccine is a mineral oil emulsion vaccine.

### Claim 14 (depends on 1)

The method of claim 1 wherein the vaccine is administered at a dose of from about 50 microliters to about 500 microliters.

### Claim 15 (depends on 1)

The method of claim 1 wherein the hatchability of the egg is at least about 85%.

### Claim 16 (depends on 1)

The method of claim 1 wherein said vaccine is in a concentration of from about 1.times. to about 10.times..