Method and device for diagnosing and distinguishing chest pain in early onset thereof
AssigneeSpectral Diagnostics, Inc.
InventorGeorge Jackowski
Life of this patent
Estimating the term…
Abstract
A diagnostic test, and a device for conducting the test, for assessing whether patient chest pain is cardiac in origin and for differentiating between unstable angina and myocardial infarction as a cause of patient chest pain is described. The diagnostic test comprises simultaneously detecting at least three selected cardiac markers with the use of at least three different monoclonal or polyclonal antibody pairs, each member of which is complementary to a different marker, which is released by heart muscle at varying stages after the onset of chest pain and is indicative of the cause of the chest pain.
Claims25
A method for determining if a patient presenting with chest pain is undergoing a cardiac event and, if so, if the cardiac event is an unstable angina or a myocardial infarction, comprising
- a. detecting the presence of increased levels of at least three different markers of cardiac damage present in a blood, serum, or plasma sample from the patient after onset of chest pain, wherein:
- i. a first marker is an ischemic marker;
- ii. a second marker is released from cardiac tissue only as a result of myocardial infarction;
- and
- iii. a third marker is a cardiac-specific ischemic marker;
- by contacting the sample with three antibodies, one of which one is specific for each of the markers, wherein one of the markers to which the antibodies bind is released before about six hours after the onset of chest pain and detecting binding of the antibody to the marker, whereby the presence of increased levels of a marker in the sample is indicated by binding of the marker with the respective antibody;
- and
- b. correlating the presence or absence of increased levels of each of the three markers with the presence or absence of unstable angina or myocardial infarction.
A diagnostic kit for determining if a patient presenting with chest pain is undergoing a cardiac event and, if so, if the cardiac event is an unstable angina or a myocardial infarction, said kit comprising a container with at least three labeled antibodies specific for each of three different markers, wherein the antibodies are selected so that:
- a. the first antibody binds to an ischemic marker;
- b. the second antibody binds to a marker released from cardiac tissue only as a result of myocardial infarction;
- and
- c. the third antibody binds to a cardiac-specific ischemic marker;
- wherein one of the markers to which the antibodies bind is released before about six hours after the onset of chest pain.
A diagnostic kit for determining if a patient presenting with chest pain is undergoing a cardiac event and, if so, if the cardiac event is an unstable angina or a myocardial infarction, said kit comprising at least three antibodies specific for each of three different markers irreversibly immobilized on a solid phase support, wherein the antibodies are selected so that:
- a. the first antibody binds to an ischemic marker;
- b. the second antibody binds to a marker released from cardiac tissue only as a result of myocardial infarction;
- and
- c. the third antibody binds to a cardiac-specific ischemic marker;
- wherein one of the markers to which the antibodies bind is released before about six hours after the onset of chest pain;
- and
- further comprising three different labeled markers which bind to each of the antibodies.
Dependencies are inferred from the claim text.