Back to results
US 5,747,268 AGranted
Tumor marker control
AssigneeDade International Inc.
InventorsKathryn Herring, Denise Sandberg
Life of this patent
Estimating the term…
Abstract
The present invention provides a stable control serum or plasma for tumor diagnosis, wherein the control contains the tumor markers relevant for the diagnosis of tumors. The serum or plasma has a reduced lipid content. The present invention also provides for a method of making the control.
Claims13
A control for the determination of tumor markers comprising a mixture of:
- (a) a base material comprising human serum or plasma having reduced lipids;
- (b) a plurality of tumor markers said tumor markers comprising at least PSA and ACTH wherein the PSA and ACTH in the control each have a reconstituted stability of at least thirty days at about -20 C.
A control for the determination of tumor markers comprising a mixture of:
- (a) a base material comprising human serum or plasma having reduced lipids;
- (b) a plurality of tumor markers said tumor markers comprising PSA and consisting of at one of the following additional tumor markers selected from gastrin, gamma enolase, CA 15-3, CA 549, and LD-1 wherein the PSA in the control has a reconstituted stability of at least thirty days at about -20 C.
A method to separate CA 125 from TPA comprising:
- a) adding a fluid containing CA 125 and TPA to a hydrophobic interaction chromatography media in the presence of a phosphate buffer at about physiological pH;
- and
- b) collecting the eluant.
A method to separate CEA and/or CA 19-9 from TPA comprising:
- a) adding a fluid containing CEA and/or CA 19-9 and TPA to a hydrophobic interaction chromatography media in the presence of a phosphate buffer at about physiological pH;
- and
- b) collecting the eluant.
A method to separate TPA from CEA, CA 19-9 or CA 1 25 comprising:
- a) adding a fluid containing TPA to a hydrophobic interaction media in the presence of a phosphate buffer at about physiological pH said buffer also containing between at least 2.5M urea to less than 6M urea;
- and
- b) washing the chromatography media with said buffer;
- c) washing the chromatography media with at least about 6M urea;
- and
- d) collecting the eluant.
3 more elements
Dependencies are inferred from the claim text.