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US 5,744,335 AGranted
Process of transfecting a cell with a polynucleotide mixed with an amphipathic compound and a DNA-binding protein
AssigneeMiRus LLC
InventorsJon A. Wolff, Jeffery Fritz, Vladimir Budker, James Hagstrom
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Abstract
The present invention provides a process of transfecting a cell with a polynucleotide mixed with one or more amphipathic compounds and an effective amount of a DNA-binding protein. Exemplary and preferred DNA-binding proteins are H1, H2A, and H2B. Exemplary and preferred amphipathic compounds are cationic amphipathic compounds.
Claims15
A process of transfecting a polynucleotide into a cell comprising:
- associating a selected cell with an amphipathic compound, an effective amount of a polynucleotide-binding protein, the polynucleotide-binding protein is selected from the group consisting of histone and histone with a nuclear localizing signal, and a selected polynucleotide, in solution, wherein encapsulation of the polynucleotide by the amphipathic compound is not required for transfection.
Dependencies are inferred from the claim text.