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US 5,744,305 AGranted
Arrays of materials attached to a substrate
AssigneeAffymetrix, Inc.
InventorsStephen P.A. Fodor, Lubert Stryer, J. Leighton Read, Michael C. Pirrung
Life of this patent
Estimating the term…
Abstract
A synthetic strategy for the creation of large scale chemical diversity. Solid-phase chemistry, photolabile protecting groups, and photolithography are used to achieve light-directed spatially-addressable parallel chemical synthesis. Binary masking techniques are utilized in one embodiment. A reactor system, photoremovable protective groups, and improved data collection and handling techniques are also disclosed. A technique for screening linker molecules is also provided.
Claims26
An array of oligonucleotides, the array comprising:
- a planar, non-porous solid support having at least a first surface;
- and
- a plurality of different oligonucleotides attached to the first surface of the solid support at a density exceeding 400 different oligonucleotides/cm.sup.2, wherein each of the different oligonucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
An array of polynucleotides, the array comprising:
- a planar non-porous solid support having at least a first surface;
- and
- a plurality of different polynucleotides attached to the first surface of the solid support at a density exceeding 400 different polynucleotides/cm.sup.2, wherein each of the different polynucleotides is attached to the surface of the solid support in a different predefined region, has a different determinable sequence, and is at least 4 nucleotides in length.
Dependencies are inferred from the claim text.