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US 5,741,668 AGranted
Expression of a gene for a modified green-fluorescent protein
AssigneesRutgers, The State University of New Jersey, The Trustees of Columbia University in the City of NY
InventorsWilliam W. Ward, Martin Chalfie
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Abstract
A pre-coelenterazine peptide comprising a modified A. victoria GFP having an amino acid sequence in which Ser.sup.65 is replaced with Tyr. There is further provided a polynucleotide encoding the pre-coelenterazine peptide, allowing synthesis of large, pure amounts of coelenterazine.
Claims7
A method of synthesizing coelenterazine comprising:
- a) synthesizing a pre-coelenterazine peptide comprising a modified amino acid sequence of a green-fluorescent protein of A. victoria in which R.sup.65 is Tyr, said method comprising
- incubating a polynucleotide in the presence of means for effecting expression of said polynucleotide under conditions favorable to expression of said polynucleotide,
- said polynucleotide comprising one or more sequences of nucleotide bases collectively encoding said pre-coelenterazine peptide, wherein said one or more sequences of bases collectively encoding said amino acid sequence of said pre-coelenterazine peptide are uninterrupted by non-coding sequences;
- and
- b) isolating coelenterazine from said means.
2 more elements
Dependencies are inferred from the claim text.