Mutanase-containing oral compositions
AssigneeLion Corporation
InventorsYoshio Asai, Motoyasu Ohdera, Hiromitsu Kigawa, Isao Shimotsuura, Yoshiko Yokobori, Masanori Hirano, Koji Shibuya
Life of this patent
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Abstract
An oral composition contains mutanase prepared from a culture which is obtained by cultivating a mutanase-producing microorganism belonging to the genus Bacillus having negative protease producibility. The oral composition is effective for suppressing dental plaque formation while the mutanase possesses commercially acceptable stability in the oral composition.
Claims9
An oral composition comprising mutanase having the following physicochemical properties (1) to (9):
- (1) action:
- an ability to cleave .alpha.-1,3-glucosidic links of mutan,
- (2) substrate specificity:
- an ability to effectively decompose mutan,
- (3) optimum pH:
- pH 4 to 4.5 when reacting on a mutan substrate at 35.degree. C. for 10 minutes,
- (4) pH range for stability:
- pH 4 to 10 when kept at 25.degree. C. for 24 hours,
- (5) optimum temperature:
- 50.degree. to 65.degree. C. when reacted at pH 5 with mutan as a substrate,
- (6) thermal stability:
- enzyme activity remains stable below 50.degree. C. after incubation at pH 5 for 10 minutes,
- (7) effect of metal ions:
- mercury and silver show inhibitory effect on a mutan substrate,
- (8) effect of inhibitors:
- p-chloromercurybenzoic acid shows inhibitory effect on a mutan substrate, and
- (9) molecular weight:
- about 140,000 to about 160,000 as determined by SDS-polyacrylamide gel electrophoresis.
An oral composition comprising dextranase and mutanase prepared from a culture which is obtained by cultivating a mutanase-producing microorganism belonging to the genus Bacillus having negative protease producibility,
- said oral composition comprising mutanase having the following physicochemical properties (1) to (9):
- action:
- an ability to cleave .alpha.-1,3-glucosidic links of mutan,
- (2) substrate specificity:
- an ability to effectively decompose mutan,
- (3) optimum pH:
- pH 4 to when reacting on a mutan substrate at 35.degree. C. for 10 minutes,
- (4) pH range for stability:
- pH 4 to 10 when kept at 25.degree. C. for 24 hours,
- (5) optimum temperature:
- 50.degree. to 65.degree. C. when reacted at pH 5 with mutan as a substrate,
- (6) thermal stability:
- enzyme activity remains stable below 50.degree. C. after incubation at pH 5 for 10 minutes,
- (7) effect of metal ions:
- mercury and silver show inhibitory effect on a mutan substrate,
- (8) effect of inhibitors:
- p-chloromercurybenzoic acid shows inhibitory effect on a mutan substrate, and
- (9) molecular weight:
- about 140,000 to about 160,000 as determined by SDS-polyacrylamide gel electrophoresis.
Dependencies are inferred from the claim text.