Antisense oligonucleotides specific for the muscarinic type 2 acetylcholine receptor MRNA
OwnerRachel L. Galli, Harris R. Lieberman +1inventors
InventorsRachel L. Galli, Harris R. Lieberman, Richard E. Fine
Life of this patent
Estimating the term…
Abstract
Antisense oligonucleotides specific for the muscarinic type 2 acetylcholine receptor mRNA have been identified. Administration of these oligonucleotides to animals resulted in an increase in memory and learning. Methods for discovering other oligonucleotides with the same activity are taught, as are uses of the antisense molecules for treatment of human and animal diseases.
Claims19
An antisense nucleic acid molecule comprising a segment complementary to a sequence unique to muscarinic type 2 receptor RNA, wherein when administered to a living organism, said antisense molecule is capable of showing a behavioral change.
A composition comprising an antisense muscarinic type 2 acetylcholine receptor oligonucleotide selected from the group consisting of 5'-AA CTG CCA GAA GAG AAT G-3' (SEQ. ID. 1), 5'- AGG TCT CTG TGT TAG CAT-3' (SEQ. ID. 2), 5'- CAG GCA TAT TGT TAT TGT-3' (SEQ. ID. 3), and 5'-TAG CCT CTG TGT TAG GAT-3' (SEQ ID NO:4), which composition reduces muscarinic type 2 acetylcholine receptor binding in cells in vitro.
Dependencies are inferred from the claim text.