Method for selective inactivation of viral replication
AssigneeRiboGene, Inc.
InventorsVincent J. Miles, Michael B. Mathews, Michael G. Katze
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Abstract
Method for screening for an antiviral agent, by determining whether a potential agent interacts with a virus or cellular component which allows or prevents preferential translation of a virus RNA compared to a host RNA under virus infection conditions; and determining whether any interaction of the agent with the component reduces the level of translation of an RNA of the virus.
Claims40
A method for screening for a potential antiviral agent, comprising the steps of:
- determining whether a potential antiviral agent interacts with a virus or cellular component which allows or prevents preferential translation of a virus RNA compared to a host RNA under virus infection conditions;
- and
- determining whether any interaction of said agent:
- with said component reduces the level of translation of a RNA of said virus.
The method of 7, wherein said incubating is carried out in solution phase, and said examining includes passing the mixture through a filter which retains said IRES element only when the element is bound to the cellular or viral translation initiation protein.
A method for screening for a potential antiviral agent, comprising the steps of:
- determining whether said agent is effective to inhibit viral replication in a host eukaryotic cell, where the virus produces an inhibitor which interferes with the activation or activity of the host-cell interferon-induced, double-stranded RNA-activated protein kinase, comprising
- incubating a mixture containing the protein kinase, the viral inhibitor, and the agent to be tested under conditions effective to cause inhibitor interference with the activation or activity of the protein kinase, and
- examining the mixture for such interference.
A method for screening for a potential antiviral agent, comprising the steps of:
- determining whether said agent is effective to inhibit viral replication in a host eukaryotic cell, where the host cell produces an inhibitor which interferes with the activation of the host-cell interferon-induced, double-stranded RNA-activated protein kinase, comprising
- incubating a mixture containing the protein kinase, the host cell inhibitor, and the agent to be tested under conditions effective to cause inhibitor interference with the activation of the protein kinase, and
- examining the mixture for such interference.
A yeast cell genetically engineered to express:
- (a) a gene encoding a mammalian interferon-induced, double-stranded RNA-activated protein kinase,
- (b) a reporter gene whose expression is increased by activation of the protein kinase, and
- (c) a viral gene for producing a viral inhibitor able to block activation of the protein kinase.
A yeast cell genetically engineered to express:
- (a) a gene encoding a mammalian interferon-induced, double-stranded RNA-activated protein kinase,
- (b) a reporter gene whose expression in increased by activation of the protein kinase, and
- (c) a gene encoding a mammalian protein induced or activated by a virus, which blocks activation of the mammalian interferon-induced, double-stranded RNA-activated protein kinase.
Dependencies are inferred from the claim text.