Recombinant endothelin converting enzyme-2 and its use in ECE inhibitor screening
AssigneeBoard of Regents, The University of Texas System
InventorMasashi Yanagisawa
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Abstract
Disclosed is a nucleic acid segment encoding endothelin converting enzyme-2 (ECE-2), that produces mature ET-1 from big ET-1 both in vitro and in transfected cells. Also disclosed is a polypeptide composition comprising partially purified ECE-2 and methods of using ECE-2 to screen candidate substances as inhibitors of ECE-2. Methods of producing recombinant ECE-2 are also disclosed.
Claims7
A composition comprising a partially purified endothelin converting enzyme-2 (ECE-2) polypeptide, having an activity of at least about 275 fmol/30 min/20 .mu.g at pH 5.6-5.6.
An antigenic composition comprising the C-terminal region of endothelin converting enzyme-2.
Dependencies are inferred from the claim text.