Assay reagents
AssigneeAlusuisse Holdings A.G.
InventorsElizabeth Anne Weavers, Michael Joseph Powell
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Abstract
The present invention relates to a peroxidase-containing reagent comprising a buffered aqueous solution of a peroxidase conjugate and a substrate for the peroxidase, in the absence of peroxide. The invention further relates to a method of stabilizing the peroxidase activity of a buffered aqueous solution containing a peroxidase conjugate. Further, the invention relates to a tetramethylbenzidine peroxidase substrate reagent and to a method of preparing same.
Claims6
A peroxidase-containing reagent comprising a buffered aqueous solution comprising a peroxidase conjugate and a specific substrate for the peroxidase, in the absence of peroxide.
A method for stabilizing the peroxidase activity of a buffered aqueous solution containing a peroxidase conjugate, comprising adding to the solution, an amount of a specific substrate for the peroxidase, in the absence of peroxide, effective to stabilize the peroxidase activity of the solution.
An assay reagent kit comprising a first buffered aqueous solution comprising a peroxidase or a peroxidase-conjugate and a specific substrate for the peroxidase or peroxidase-conjugate in the absence of peroxide, and a second aqueous solution comprising tetramethylbenzidine and an amount of .beta.-cyclodextrin sufficient to solubilize the tetramethylbenzidine.
Dependencies are inferred from the claim text.