US 5,736,342 A · Biosensor for detecting the presence of chosen analytes

Abstract

The present invention relates to a biosensor for detecting the presence of an analyte in solution. The biosensor is composed of: a) an ion channel receptor immobilized in a suspended lipid film which is in contact with an ion-containing solution on each side thereof; b) a ligand, conjugated to the receptor, which has particular affinity for the analyte in solution, and, which, upon binding to the analyte in solution, causes an inhibition in ion flow through the channel; and c) means to detect the inhibition of ion flow through the channel in response to the binding of conjugated ligand and analyte.

Claims18

Dependent claims

A biosensor for use in a non-competitive immunoassay for detecting the presence of a chosen analyte in solution which comprises:

a) an ion channel receptor immobilized in a suspended lipid film which film is in contact with an ion-containing solution on each side thereof;
b) a ligand, conjugated to the receptor, said ligand which specifically binds to the analyte in solution, and, which, upon binding to the analyte in solution, causes an inhibition in ion flow through the ion channel;
and
c) means to detect the inhibition of ion flow through the ion channel in response to the binding of conjugated ligand and analyte.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

08/555,347Filed 8 Nov 1995 · continuation

Priority

21 Sep 1993

Granted

7 Apr 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

G01N 33/944
Neurotransmitters: acetylcholine
G01N 33/54373
Apparatus specially adapted for solid-phase testing: involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
G01N 33/5438
Involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings: electrodes
G01N 33/566
Immunoassay; Biospecific binding assay; Materials therefor: using specific carrier or receptor proteins as ligand binding reagents where possible…

Biosensor for detecting the presence of chosen analytes

Life of this patent

Biosensor for detecting the presence of chosen analytes

Life of this patent

Abstract

Claims18