Methods and test kits for specific and sensitive diagnosing of periodontal diseases
AssigneeOy Medix Biochemica AB
InventorsTimo Arto Sorsa, Sari Hannele Tikanoja, Leila Christina Lundqvist
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Abstract
Methods and test kits are described which provide a reliable, sensitive and selective assessment of periodontal disease activity, peri-implantitis or HIV(+)-infection/AIDS-disease related periodontal diseases. The preferred methods and test kits are constructed to be easy and rapid chair-side tests. The method is based on the preparation and use of monoclonal antibodies which recognize the active mammalian matrix metalloproteinase-S (MMP-8) and is capable of differentiating between said active matrix metalloproteinase-8 and its inactive proform.
Claims24
A test kit for diagnosing periodontal disease, peri-implantitis or HIV(+)-infection/AIDS-disease related periodontal diseases comprising at least one detectable label and at least one monoclonal antibody of which at least one specifically recognizes a tissue destructive active form of mammalian matrix metalloproteinase-8 (MMP-8) in gingival crevicular fluid, saliva or mouthrinse samples, said at least one monoclonal antibody being directed against a part of said MMP-8 which is exposed during activation of said MMP-8 from the proenzyme form, and wherein an increased level of MMP-8 indicates periodontal disease.
A method for diagnosing periodontal disease, peri-implantitis or HIV(+)-infection/AIDS-disease related periodontal diseases, wherein the detection is performed as an immunological assay comprising the steps of:
- (a) collecting a gingival crevicular fluid sample with a sampling device;
- (b) contacting said sample with at least one monoclonal antibody, which recognizes a tissue destructive active form of mammalian matrix metalloproteinase-8 in gingival crevicular fluid, saliva or mouthrinse samples, said monoclonal antibodies being directed against a part of said MMP-8 which is exposed during activation of said MMP-8 from the proenzyme form;
- and
- (c) detecting the presence of said active mammalian matrix metalloproteinase-8, wherein an increased level of MMP-8 indicates periodontal disease.
A method for prescreening for the risk of periodontal disease, peri-implantitis or HIV(+)-infection/AIDS-disease related periodontal diseases, wherein the detection is performed as an immunological assay comprising the steps of:
- (a) collecting a sample containing gingival crevicular fluid;
- (b) contacting said sample with at least one monoclonal antibody, which recognises a tissue destructive form of mammalian matrix metalloproteinase-8 in gingival crevicular fluid, saliva or mouthrinse sample, said monoclonal antibody being directed against a part of said MMP-8 which is exposed during activation of said MMP-8 from the proenzyme form;
- and
- (c) detecting the presence of said mammalian matrix metalloproteinase-8 by the aid of an immunological method capable of recognizing an increased level of matrix metalloproteinase-8 wherein an increased level of MMP-8 indicates a risk for said diseases.
Dependencies are inferred from the claim text.