Method of promoting adhesion between tissue surfaces
AssigneeOrthogene, Inc.
InventorsKay Juergensen, Daniel Aeschlimann, Ernst B. Hunziker
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Abstract
A formulated biological adhesive composition utilizes tissue transglutaminase in a pharmaceutically acceptable aqueous carrier. The tissue transglutaminase is used in an effective catalytic amount to promote adhesion between tissue surfaces upon treatment thereof by catalyzing the reaction between glutaminyl residues and amine donors of the tissue and/or the enzyme. The carrier contains a divalent metal ion such as calcium to promote said reaction.
Claims16
A method of promoting adhesion upon treatment of tissue comprising
- providing tissue for adhesion, and
- applying to said tissue an effective amount of tissue transglutaminase to promote adhesion of said tissue in the presence of a divalent metal ion selected from the group consisting of calcium and strontium in an amount in the range of about 0.5 mM to about 100 mM at a pH of about 7.0 to about 8.5.
A method of treatment of biomatrix implants which further containing a naturally occurring or synthetic protein having transglutaminase substrate sites comprising:
- providing tissue for adhesion, and
- applying to said tissue an effective amount of tissue transglutaminase in a pharmaceutically acceptable aqueous carrier to promote adhesion of said tissue in the presence of a divalent metal ion selected from the group consisting of calcium and strontium in an amount in the range of about 0.5 mM to about 100 mM at a pH of about 7 to about 8.5.
Dependencies are inferred from the claim text.