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US 5,733,745 AGranted
Bovine heat shock promoter and uses thereof
AssigneeBiostar, Inc.
InventorsJacek Kowalski, Scott Gilbert, Timothy J. Zamb
Life of this patent
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Abstract
A novel expression system using the heat-inducible bovine hsp70A promoter and associated cis-acting elements is disclosed. The system provides for the continuous production of a highly pure, authentic protein, substantially free of infectious viral and cellular protein contaminants.
Claims8
A method of producing a recombinant polypeptide comprising:
- (A) transforming a Madin-Darby bovine kidney (MDBK) cell with a recombinant expression construct comprising:
- (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide;
- and
- (b) hsp70 control sequences operably linked to said nucleic acid molecule, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise
- (i) a bovine hsp70A promoter selected from the group consisting of (1) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 350 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NOS:1-2);
- (2) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 265 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NOS:1-2);
- (3) a promoter which comprises the nucleotide sequence depicted at nucleotide positions 1 to 441, inclusive, of the upper strand of FIG. 2 (SEQ ID NOS:1-2);
- and (4) a promoter substantially homologous and functionally equivalent to (1), (2) or (3);
- (ii) a bovine 5'-untranslated region positioned downstream of said promoter, and
- (iii) a 3'-untranslated region positioned downstream of said coding sequence;
- and
- (B) treating said transformed cell with heat, under conditions whereby said coding sequence for said selected polypeptide is expressed, thereby producing said recombinant polypeptide.
9 more elements
A method of producing a recombinant polypeptide comprising:
- (A) transforming a Madin-Darby bovine kidney (MDBK) cell with a recombinant expression construct comprising:
- (a) a nucleic acid molecule that comprises a coding sequence for a selected polypeptide;
- and
- (b) hsp70 control sequences operably linked to said nucleic acid molecule and a 3'-untranslated region positioned downstream of said coding sequence, whereby said coding sequence will be transcribed and translated when in a host cell to produce said selected polypeptide, and at least one of said control sequences is heterologous to said coding sequence, wherein said hsp70 control sequences comprise the nucleotide sequence depicted at nucleotide positions 1 to 666, inclusive, of the upper strand of FIG. 2 (SEQ ID NOS:1-2);
- (B) treating said transformed cell with heat, under conditions whereby said coding sequence for said selected polypeptide is expressed, thereby producing said recombinant polypeptide.
2 more elements
Dependencies are inferred from the claim text.