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AssigneesCambridge Antibody Technology Limited, Medical Research Council
InventorsKevin Stuart Johnson, Gregory Paul Winter, Andrew David Griffiths, Andrew John Hammond Smith, Peter Michael Waterhouse
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Methods, recombinant host cells and kits are disclosed for the production of members of specific binding pairs (sbp), e.g. antibodies, using display on the surface of secreted replicable genetic display packages (rgdps), e.g. filamentous phage. To produce a library of great diversity, recombination occurs between first and second vectors comprising nucleic acid encoding first and second polypeptide chains of sbp members respectively, thereby producing recombinant vectors each encoding both a first and a second polypeptide chain component of an sbp member. The recombination may take place in vitro or intracellularly and may be site-specific, e.g. involving use of the loxP sequence and mutants thereof. Recombination may take place after prior screening or selecting for rgdps displaying sbp members which bind complementary sbp member of interest.
A method of producing specific binding pair members comprising a first polypeptide chain and a second polypeptide chain, which method comprises:
A population of rgdps each rgdp in said population displaying at its surface a sbp member and each containing nucleic acid which comprises a combination of (i) nucleic acid encoding a first polypeptide chain of an sbp member and (ii) nucleic acid encoding a second polypeptide chain of an sbp member, the population containing 10.sup.10 or more combinations of (i) and (ii) produced by site-specific recombination.
Dependencies are inferred from the claim text.