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US 5,733,727 AGranted
Myocardial grafts and cellular compositions
AssigneeIndiana University Foundation
InventorLoren J. Field
Life of this patent
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Abstract
Described are preferred myocardial grafts of skeletal myoblasts or cardiomyocytes, and cellular compositions and methods useful in obtaining the grafts. The myocardial grafts are stable and can be used, for example, to deliver recombinant proteins directly to the heart.
Claims13
A method of obtaining a population of cells, comprising:
- transfecting embryonic stem cells to introduce a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells;
- causing the stem cells to differentiate in culture;
- and
- selecting said cardiomyocyte cell lineage based on said marker gene.
1 more element
An embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells.
A method of obtaining a population of cells, comprising:
- providing an embryonic stem cell having introduced DNA encoding a marker gene enabling selection of a cardiomyocyte cell lineage from other cell lineages resulting from the differentiation of the stem cells;
- causing the stem cell to differentiate in culture to form a mixed differentiated cellular population containing said a cardiomyocyte cell lineage and said other cell lineages;
- selecting from the cellular population said a cardiomyocyte cell lineage from said other cell lineages based on said marker gene.
Dependencies are inferred from the claim text.