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US 5,731,149 AGranted
Antibiotic cryptdin peptides and methods of their use
AssigneeThe Shriner's Hospital for Crippled Children
InventorsMichael E. Selsted, Andre J. Ouellette
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AssigneeThe Shriner's Hospital for Crippled Children
InventorsMichael E. Selsted, Andre J. Ouellette
Estimating the term…
The present invention provides substantially purified cryptdin peptides having a consensus amino acid sequence: X.sub.1 --C--X.sub.2 --C--R--X.sub.3 --C--X.sub.4 --E--X.sub.5 --C--X.sub.6 --C--C--X.sub.7 wherein X.sub.1 is 3 to 9 amino acids; X.sub.2 is one amino acid, preferably Y, H or R; X.sub.3 is 2 or 3 amino acids; X.sub.4 is three amino acids; X.sub.5 is five amino acids; X.sub.6 is 6 to 10 amino acids; and X.sub.7 is 0 to 9 amino acids. The invention also provides a substantially purified mouse cryptdin having a consensus amino acid sequence: X.sub.1 --L--X.sub.2 --C--Y--C--R--X.sub.3 --C--K--X.sub.4 --E--X.sub.5 --G--T--C--X.sub.6 --C--C--X.sub.7 wherein X.sub.1 is 3 or 4 amino acids, preferably LRD, LSKK (SEQ ID NO: 1) or LRG; X.sub.2 is 1 amino acid, preferably V, L or I; X.sub.3 is 3 amino acids, preferably KGH or *RG, where * is S, T, K, I or A; X.sub.4 is 2 amino acids, preferably GR, RR or RG; X.sub.5 is 3 amino acids, preferably RMN, RVR, RVF HMN or HIN; X.sub.6 is 6 to 9 amino acids, preferably GIRFLY (SEQ ID NO: 2) or RNLFLTFVF (SEQ ID NO: 3), RRGHLMYTL (SEQ ID NO: 4) or RKGHL*YT* (SEQ ID NO: 5), where * independently is L or M; and X.sub.7 is 0 to 3 amino acids, preferably R, S or PRR. The invention also provides cryptdin analogs, devoid of one or more amino acids N-terminal to the first cysteine. In addition, the invention provides nucleic acid molecules encoding cryptdin peptides. The invention further provides methods for detecting inflammatory pathologies in a subject and for treating an inflammatory pathology in a subject by administering a pharmaceutical composition containing a cryptdin peptide.
A substantially purified nucleic acid molecule encoding a cryptdin.
A substantially purified nucleic acid molecule comprising a cryptdin gene.
A substantially purified nucleic acid molecule, comprising a gene selected from the group consisting of the mouse cryptdins 1, 2, 3, 5, 6 and i genes whose sequences are shown in FIG. 11 (SEQ ID NOS:
A substantially purified nucleic acid molecule, comprising a gene selected from the group consisting of rat cryptdins 1, 2 and 3 genes whose sequences are shown in FIGS. 15A to 15C (SEQ ID NOS:
A substantially purified nucleic acid molecule comprising a cDNA encoding a cryptdin.
A nucleic acid molecule, comprising a cDNA selected from the group consisting of mouse cryptdins 1-17 cDNAs whose sequences are shown in FIG. 10 (SEQ ID NOS:
A substantially purified nucleic acid molecule, comprising a cDNA selected from the group consisting of the mouse cryptdins 2-17 cDNAs whose sequences are shown in FIG. 10 (SEQ ID NOS:
A substantially purified nucleic acid molecule, comprising a cDNA selected from the group consisting of the rat cryptdins 1,-2 and 3 cDNAs whose sequences are shown in FIGS. 14A to 14C (SEQ ID NOS:
A nucleotide sequence, comprising at least ten contiguous nucleotides of a nucleic acid molecule selected from the group consisting of mouse cryptdins 2-17 cDNAs whose sequences are in FIG. 10 (SEQ ID NOS:
A substantially purified nucleic acid molecule encoding a cryptdin selected from the group consisting of mouse cryptdins 2-17 cDNAs whose sequences are shown in FIG. 9A (SEQ ID NOS:
Dependencies are inferred from the claim text.