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US 5,728,559 AGranted
Separation of proteins
AssigneeNovo Nordisk A/S
InventorsBirgitte Mahler Nilsson, Mads Aage Laustsen, Anders Rancke-Madsen
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Abstract
The present invention relates to a method of separating a protein, in particular an enzyme, from an aqueous solution of proteins, comprising (a) providing an aqueous mixture of proteins with a salt concentration at or below 1.5 Molar, to which a water soluble polymer has been added, and (b) recovery of the protein on crystalline form.
Claims19
A method for obtaining a crystallized microbial protein from an aqueous mixture of proteins, said method comprising of the steps of:
- (a) obtaining a microbial culture broth having a mixture of proteins, comprising a desired protein selected from the group consisting of an oxidoreductase, a protease, a lipase, a cellulase and an amylase;
- (b) adjusting the salt concentration of said protein mixture at or below 1.5 Molar;
- (c) adding a water soluble polymer to the protein mixture of step (b);
- (d) stirring the polymer-containing protein mixture of step (c) for up to 48 hrs;
- and
- (e) recovering the desired protein in crystalline form from the stirred mixture of step (d).
3 more elements
Dependencies are inferred from the claim text.