US 5,728,553 A · High purity albumin and method of producing

Abstract

A process for the preparation of albumin which has extremely low levels of or is essentially free of colorants, metal ions, human proteins, fragments of albumin, polymers or aggregates of albumin, and viruses, and which is relatively non-glycated, relatively high in free thiol and with an intact C-terminus. The process comprises passing albumin (preferably expressed and secreted by transformed yeast) through two chromatography purifications, ultrafiltering the product, passing through two further chromatography steps and again ultrafiltering the product.

Claims12

Dependent claims

A process for preparing an albumin product at least 99.9% pure an albumin-containing solution, comprising the steps of:

(a) passing the albumin-containing solution through a cation exchange matrix under conditions such that the albumin will bind to the matrix;
(b) eluting from the matrix an albumin-containing cation exchange eluate;
(c) passing the cation exchange eluate through an anion exchange matrix under conditions such that the albumin will bind to the matrix;
(d) eluting from the anion exchange matrix an albumin-containing anion exchange eluate;
(e) passing the anion exchange eluate through an affinity matrix comprising an albumin-binding compound;
(f) eluting from the affinity matrix an albumin-containing affinity matrix eluate;
(g) passing the affinity matrix eluate through a gel permeation matrix to obtain a fraction enriched in albumin.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application

08/378,859Filed 25 May 1995 · continuation in part

Priority

23 Sep 1992

Granted

17 Mar 1998

Term ends

Not estimatedThe source records don’t support an estimate.

Classification7 codes

C07K 14/70571
Receptors; Cell surface antigens; Cell surface determinants: for neuromediators, e.g. serotonin receptor, dopamine receptor
A61P 7/08
Drugs for disorders of the blood or the extracellular fluid: plasma substitutes; Perfusion solutions; Dialytics or haemodialytics; Drugs for…
A61P 17/02
Drugs for dermatological disorders: for treating wounds, ulcers, burns, scars, keloids, or the like
C07K 14/765
Albumins: serum albumin, e.g. HSA

High purity albumin and method of producing

Life of this patent

High purity albumin and method of producing

Life of this patent

Abstract

Claims12