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US 5,726,038 AGranted
DNA construct encoding the YAP3 signal peptide
AssigneeNovo Nordisk A/S
InventorsLars Christiansen, Jens Gunner Litske Petersen
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Abstract
A DNA construct comprising the following sequence: 5'-P-SP-(LP).sub.n -PS-HP-3' wherein P is a promoter sequence, SP is a DNA sequence encoding the yeast aspartic protease 3 (YAP3) signal peptide, LP is a DNA sequence encoding a leader peptide, n is 0 or 1, PS is a DNA sequence encoding a peptide defining a yeast processing site, and HP is a DNA sequence encoding a polypeptide which is heterologous to a selected host organism. The YAP3 signal peptide provides efficient secretion of heterologous proteins in yeast.
Claims16
A DNA construct comprising the following sequence
- 5'-P-SP- (LP).sub.n -PS-HP-3'
- wherein
- P is a promoter sequence,
- SP is a DNA sequence encoding the yeast aspartic protease 3 (YAP3) signal peptide,
- LP is a DNA sequence encoding a leader peptide, n is 0 or 1,
- PS is a DNA sequence encoding a peptide defining a yeast processing site, and
- HP is a DNA sequence encoding a polypeptide which is heterologous to a selected host organism.
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Dependencies are inferred from the claim text.