Back to results
US 5,723,584 AGranted
Biotinylation of proteins
AssigneeAffymax Technologies N.V.
InventorPeter J. Schatz
Life of this patent
Estimating the term…
Abstract
Biotinylation peptides can be fused to other peptides or proteins of interest using recombinant DNA techniques to provide efficient methods for biotinylating the resulting fusion proteins in vivo or in vitro.
Claims6
A biotinylation peptide comprising:
- a non-naturally occurring peptide sequence of 13 to 50 amino add residues comprising the sequence:
- Leu Xaa.sub.1 Xaa.sub.2 Ile Xaa.sub.3 Xaa.sub.4 Xaa.sub.5 Xaa.sub.6 Lys Xaa.sub.7 Xaa.sub.8 Xaa.sub.9 Xaa.sub.10 (SEQ ID NO:1),
- where
- Xaa.sub.1 is any amino acid;
- Xaa.sub.2 is any amino acid other than Leu, Val, Ile,
- Trp, Phe, or Tyr;
- Xaa.sub.3 is Phe or Leu;
- Xaa.sub.4 is Glu or Asp;
- Xaa.sub.5 is Ala, Gly, Ser, or Thr;
- Xaa.sub.6 is Gln or Met;
- Xaa.sub.7 is Ile, Met, or Val;
- Xaa.sub.8 is Glu, Leu, Val, Tyr, or Ile;
- Xaa.sub.9 is Trp, Tyr, Val, Phe, Leu, or Ile;
- and
- Xaa.sub.10 is any amino add other than Asp or Glu,
- wherein said biotinylation-peptide is capable of being biotinylated by a biotin ligase at the lysine residue adjacent to Xaa.sub.6.
13 more elements
Dependencies are inferred from the claim text.