Thermostable trehalose-releasing enzyme, and its preparation and uses
AssigneeKabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo
InventorsShouji Ikegami, Michio Kubota, Toshiyuki Sugimoto, Toshio Miyake
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Abstract
Disclosed are novel thermostable trehalose-releasing enzyme, and its preparations and uses. The enzyme is obtainable from the culture of microorganisms such as Sulfolobus acidocaldarius (ATCC 33909 and ATCC 49426) and Sulfolobus solfataricus (ATCC 35091 and ATCC 35092), and capable of hydrolyzing at a temperature of over 55.degree. C. the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and having a degree of glucose polymerization of 3 or higher. Trehalose and compositions containing the same are extensively useful in food products, cosmetics and pharmaceuticals.
Claims15
A purified trehalose-releasing enzyme which specifically hydrolyses the linkage between a trehalose moiety and the remaining glycosyl moiety in a non-reducing saccharide having a trehalose structure as an end unit and a degree of glucose polymerization of at least 3 and which enzyme is capable of acting on the non-reducing saccharide at a temperature of over 55.degree. C. while retaining its activity.
Dependencies are inferred from the claim text.